EDUCATION
University of Zurich, Institute of Physiology, Zurich, Switzerland
- Ph.D. in Biochemistry and Molecular Biology, 2007
- Diploma, Biology 2003
RESEARCH EXPERIENCE
Columbia University, Department Medicine, New York, NY
Postdoctoral Research Scientist with Robert F. Schwabe, M.D., 2007-2011
Created and testing conditional knock out mice to study hepatic liver cells during liver fibrosis resolution in mice
Created and testing conditional knock out mice to study hepatic liver cells during liver fibrosis resolution in mice
- Generated transgenic mice expressing tamoxifen-inducible CreER under the control of an endogenous Vimentin promotor specific
- Hepatic stellate cell activation and deactivation was investigated by single cell PCR and genetic tracking in transgenic mouse
University of Zurich, Institute Physiology, Zurich, Switzerland
Graduate Student with Prof. Dr. Roland H. Wenger, 2003-2007
Phospholipid stimulation and downstream target identification of the PAS domain kinase PASKIN
Phospholipid stimulation and downstream target identification of the PAS domain kinase PASKIN
- Identified novel interaction targets of the mammalian cellular serine/threonine kinase PASKIN performed by yeast two-hybrid screenings and GST pull down assays
- Examined phosphorylation sides of PASKIN auto-phosphorylation and PASKIN target phosphorylation using kinase assays, mass spectrometry and site directed mutagenesis in vitro
Free University, Institute Zoology, Berlin, Germany
Master student with Prof. Dr. Klaus Hausmann, 2002/2003
Study of the morphology and morphogenesis of the ciliate Didinium nasutum by scanning electron microscopy (SEM)
Study of the morphology and morphogenesis of the ciliate Didinium nasutum by scanning electron microscopy (SEM)
- Examined spontaneous and heat shock-induced irregularities in the cell structure of Didinium nasutum
SKILLS and TECHNIQUES
- Creating microbial expressed proteins, enzymes and fusion proteins
- Isolation of RNA and analysis by transcriptional profiling with Real Time PCR and Single Cell PCR
- Genetic screening and manipulation in budding yeast
- Immunoprecipitation of protein complexes for identification of phosphorylation sites by Mass Spectrometry
- Kinase assays and translation assays
- Mammalian cell culture
- Creating transgenic mice with Cre lox technology using Bac recombineering
- Quantification of cell fate mapping
TEACHING EXPERIENCE
- Teaching Assistant in Medicine, 2004-2006